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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
17/08/2023 |
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Actualizado : |
17/08/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. |
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Afiliación : |
MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
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Título : |
Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. |
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ISSN : |
2076-2607 (electronic). |
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DOI : |
10.3390/microorganisms11071704 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). |
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Contenido : |
ABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalen... Presentar Todo |
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Palabras claves : |
Dairy calf; MDR STEC; Mortality; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Shiga toxin-producing E. coli. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.mdpi.com/2076-2607/11/7/1704/pdf
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Marc : |
LEADER 04099naa a2200289 a 4500
001 1064289
005 2023-08-17
008 2023 bl uuuu u00u1 u #d
022 $a2076-2607 (electronic).
024 7 $a10.3390/microorganisms11071704$2DOI
100 1 $aFERNÁNDEZ, M.
245 $aShiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford, Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221).
520 $aABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
653 $aDairy calf
653 $aMDR STEC
653 $aMortality
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aShiga toxin-producing E. coli
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aVIGNOLI, R.
700 1 $aBADO, I.
700 1 $aZUNINO, P.
700 1 $aUMPIÉRREZ, A.
773 $tMicroorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
17/08/2023 |
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Actualizado : |
17/08/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MONTOSSI, F.; LUZARDO, S.; DE BARBIERI, I.; BLUMETTO, O.; CIGANDA, V.; DEL CAMPO, M.; JAURENA, M.; DEVINCENZI, T.; CARRASCO-LETELIER, L.; BRITO, G.; CIAPPESONI, G.; BANCHERO, G. |
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Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OSCAR RICARDO BLUMETTO VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VERONICA SOLANGE CIGANDA BRASCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCIA DEL CAMPO GIGENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN ALEJANDRO JAURENA BARRIOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; THAIS DEVINCENZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONIDAS CARRASCO-LETELIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
O81. Livestock ruminant production systems and value chains contributing towards a more sustainable world: opportunities and challenges. [conference abstract]. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Animal - science proceedings, July 2023, Volume 14, Issue 4, Pages 595-596. https://doi.org/10.1016/j.anscip.2023.04.082 -- OPEN ACCESS. |
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ISSN : |
2772-283X |
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DOI : |
10.1016/j.anscip.2023.04.082 |
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Idioma : |
Inglés |
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Notas : |
Article history: Available online 4 August 2023, Version of Record 4 August 2023. -- Corresponding author: F. Montossi. fmontossi@inia.org.uy -- Part of special issue: 11th International Symposium on the Nutrition of Herbivores (ISNH 2023). 4-8 June 2023, Florianópolis, Brazil. (https://www.sciencedirect.com/journal/animal-science-proceedings/vol/14/issue/4 ) -- |
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Contenido : |
The anti-meat consumption trend is installed globally, where different organized and influencer collectives propose a transformation of agri-food systems and a diet practically free of red meat. The narrative is mainly based on the negative effects of meat consumption on human health, the environment, and cruelty to animals. However, evidence based on scientific information is counteracting these visions. At least 500 million people around the world are totally dependent on livestock for their livelihood and food security. Livestock ownership is also the most common form of private asset ownership in the world and forms the basis of rural community financial capital. |
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Palabras claves : |
Consumers; Ecosystems; Livestock; Producers; Science. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.sciencedirect.com/science/article/pii/S2772283X23008580/pdf
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Marc : |
LEADER 02121nam a2200337 a 4500
001 1064295
005 2023-08-17
008 2023 bl uuuu u01u1 u #d
022 $a2772-283X
024 7 $a10.1016/j.anscip.2023.04.082$2DOI
100 1 $aMONTOSSI, F.
245 $aO81. Livestock ruminant production systems and value chains contributing towards a more sustainable world$bopportunities and challenges. [conference abstract].$h[electronic resource]
260 $aAnimal - science proceedings, July 2023, Volume 14, Issue 4, Pages 595-596. https://doi.org/10.1016/j.anscip.2023.04.082 -- OPEN ACCESS.$c2023
500 $aArticle history: Available online 4 August 2023, Version of Record 4 August 2023. -- Corresponding author: F. Montossi. fmontossi@inia.org.uy -- Part of special issue: 11th International Symposium on the Nutrition of Herbivores (ISNH 2023). 4-8 June 2023, Florianópolis, Brazil. (https://www.sciencedirect.com/journal/animal-science-proceedings/vol/14/issue/4 ) --
520 $aThe anti-meat consumption trend is installed globally, where different organized and influencer collectives propose a transformation of agri-food systems and a diet practically free of red meat. The narrative is mainly based on the negative effects of meat consumption on human health, the environment, and cruelty to animals. However, evidence based on scientific information is counteracting these visions. At least 500 million people around the world are totally dependent on livestock for their livelihood and food security. Livestock ownership is also the most common form of private asset ownership in the world and forms the basis of rural community financial capital.
653 $aConsumers
653 $aEcosystems
653 $aLivestock
653 $aProducers
653 $aScience
700 1 $aLUZARDO, S.
700 1 $aDE BARBIERI, I.
700 1 $aBLUMETTO, O.
700 1 $aCIGANDA, V.
700 1 $aDEL CAMPO, M.
700 1 $aJAURENA, M.
700 1 $aDEVINCENZI, T.
700 1 $aCARRASCO-LETELIER, L.
700 1 $aBRITO, G.
700 1 $aCIAPPESONI, G.
700 1 $aBANCHERO, G.
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